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Hepatozoon sp., Leishmania sp., and Entamoeba spp., apparently not related to the clinical signs were also detected. The myriad parasites found in the intestines included nematodes ( Ancylostoma caninum, A. Braziliensis,, Molineus sp., Pterygodermatites sp., and Trichuris sp.), cestodes ( Spirometra sp.) and (acanthocephalans. To our knowledge, R. Download Kumpulan Renungan Kristen apk 1.1 and all version history for Android. Kumpulan Renungan Kristen merupakan aplikasi android yang setiap hari menyediakan renungan harian kristen baik katolik, kristen. Download Renungan Kristen apk 1.5 and all version history for Android. Enjoy millions of the latest Android apps, games, music, movies, TV, books, magazines. During 2015, we invite you every day to use “Renungan e-RH PSM” (PSM means Morning, Afternoon, and Evening) for your daily devotions. Renungan e-RH. Aug 28, 2013 - Rabu, 28 Agustus 2013. SIKAP HIDUP ORANG KRISTEN. Matius 7:1-12. Menarik untuk diperhatikan bahwa khotbah di bukit ini merupakan rangkaian. Orang Kristen harus memiliki karakter yang lebih unggul dari dunia,. Download renungan harian kristen 2013. Atlas De Parasitologia Veterinaria Pdf ReaderoVitalii was identified in C. Brachyurus for the first time. These findings emphasize the fragility of Brazilian ecosystems, especially in disturbed areas, reinforcing the necessity of efforts to preserve these areas and wild carnivores, some of which are threatened with extinction, such as the maned wolf. History In November 2013, one adult maned wolf female weighting 18 kg was rescued from a periurban house located in the Rio Acima municipality (20°5′44.732″ S and 43°47′24.263″ W) in the state of Minas Gerais (MG), southeast of Brazil. The animal had physical debilitation and pain in the right foreleg. After its rescue by the Brazilian Federal Environmental Agency (IBAMA), it was transported to a veterinary clinic. Clinical evaluation showed right elbow luxation and tick infestation. Fipronil spray 0.25% was administered. Blood sample were collected in an ethylene diamine tetraacetic acid (EDTA) tube for further molecular analyses and for preparation of blood smears. Eleven days later, the animal died and necropsy was performed at the Pathology Sector, School of Veterinary Medicine, Universidade Federal de Minas Gerais (UFMG). The study was approved by Ethics Committee for Animal Research of the UFMG under protocol 332/2013 and by the Brazilian Institute for Environment and Natural Renewable Resources (IBAMA, Belo Horizonte, MG, Brazil) under license 34633-5. Necropsy and sampling Necropsy was performed and the animal was grossly evaluated. Samples of brain, lung, trachea, heart, esophagus, stomach, intestine, kidney, bone marrow, spleen, and lymph nodes were collected in duplicate: one portion of each tissue was fixed in 10% neutral-buffered formalin for histopathology; and the other portion of the same tissue was frozen at −20 °C and stored for subsequent DNA extraction. For search for the parasites in the intestines, anatomical segments were separated by double ligatures, the contents removed and the mucosae scraped. The collected material was washed in strainers and the solid portion was fixed and preserved in acetic formaldehyde. For histopathological analysis, tissues were dehydrated using a series of increasing ethanol concentrations, cleared in xylene, and embedded in paraffin. Sections of 4 μm were obtained and stained with hematoxylin and eosin (HE) for routine histological evaluation under light microscopy (). Parasite identification in histological sections was performed according to. DNA extraction and PCR amplification DNA was extracted from helminths, organs and blood samples using Wizard ® Genomic DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer's instructions for animal tissue and whole blood, respectively. Atlas De Parasitologia Veterinaria Pdf Reader DownloadIn all PCR assays, the reaction mixture in the first round contained 7.5 μL of GoTaq ®Green Master Mix (Promega, Madison, WI, USA), 0.6 μL of a solution containing the mixed primers (10 mM) and 5.4 μL of nuclease free water. A 1.5 μL of total DNA was added to the reaction mixture to obtain a final volume of 15 μL. The reaction mixtures in the second round assays were similar, except the templates were the products from the first round PCR reactions (1.5 μL). Sets of primers used to detect the parasite species and amplifications were performed using a touchdown PCR programmed as previously (,,, ) (). |
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